If random cylinders are different temperature, it’s not a tune issue, unless you are talking about one bank vs the other. This is a mechanical/wiring...
Its actually positive 376 degrees that you would need to input.... and Lil Sick is right, you could simply disconnect the rear o2s if you don't mind...
Did you by chance have a Trinity 2 installed, along with a Tazer, and then connected your HP Tuners block by using an ODB-2 splitter... but also left...